Goat anti-CD25 / IL2RA (aa117-129) Antibody

Goat anti-CD25 / IL2RA (aa117-129) Antibody
Principle Name: CD25 / IL2RA (aa117-129)
Official Symbol: IL2RA
All Name and Symbols: IL2RA; interleukin 2 receptor, alpha; CD25; IDDM10; IL2R; TCGFR; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; OTTHUMP00000019030; OTTHUMP00000019031; OTTHUMP00000019032; TAC antigen; interleukin-2 receptor subunit alpha; p55
Accession Number(s): NP_000408.1
Peptide Sequence: DQASLPGHCREPP, is from internal region
Human Gene ID(s): 3559
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments in Human Colorectal cancer, Cerebellum, Heart and CACO-2 lysates gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the field - have a
Reference(s): Amu S, Brisslert M. Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin Dev Immunol. 2011; 2011:481948.PMID: 21941578