Goat anti-MSI2 / musashi-2 Antibody

Pep ELISA, WB

Goat anti-MSI2 / musashi-2 Antibody
Principle Name: MSI2 / musashi-2
Official Symbol: MSI2
All Name and Symbols: MSI2; musashi RNA-binding protein 2; MSI2H; RNA-binding protein Musashi homolog 2; musashi homolog 2; musashi-2
Accession Number(s): NP_620412.1
Peptide Sequence: QGTSGSANDSQHD, is from N Terminus
Human Gene ID(s): 124540
Non-Human GeneID(s): 76626 (mouse);
Important Comments: This antibody is expected to recognize isoform a (NP_620412.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Approx 35+37kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to NP_473384.1 ). Recommended conc
Reference(s): Sakakibara S, Nakamura Y, Satoh H, Okano H. Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. The Journal of neuroscience : the official journal of the Society for Neuroscience 2001 Oct 21 (20): 8091-107.PMID: 11588182