Goat anti-CD3-ZETA / CD247 Antibody

Pep ELISA, WB

Goat anti-CD3-ZETA / CD247 Antibody
Principle Name: CD3-ZETA / CD247
Official Symbol: CD247
All Name and Symbols: CD247; CD247 molecule; CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ; CD247 antigen, zeta subunit; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface gl
Accession Number(s): NP_932170.1; NP_000725.1
Peptide Sequence: KNPQEGLYNELQKD, is from internal region
Human Gene ID(s): 919
Important Comments: This antibody is expected to recognize both reported isoforms (NP_932170.1; NP_000725.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 16-17kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6kDa according to NP_000725.1). Recommended concentration: 0.1-1ug/ml.