Goat anti-ATG4A Antibody

Pep ELISA, IHC

Goat anti-ATG4A Antibody
Principle Name: ATG4A
Official Symbol: ATG4A
All Name and Symbols: ATG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); APG4A; AUTL2; APG4 autophagy 4 homolog A; AUT-like 2, cysteine endopeptidase; OTTHUMP00000062893; autophagin 2; autophagy-related cysteine endopeptidase 2
Accession Number(s): NP_443168.2; NP_840054.1
Peptide Sequence: TEENGTVNDQTFHC, is from internal region
Human Gene ID(s): 115201
Important Comments: This antibody is expected to recognize both reported isoforms (NP_443168.2; NP_840054.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in the hepatocytes. Recommended concentration, 2-4ug/ml.
Reference(s): Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A J Biol Chem. 2003 Apr 18; 278(16):14053-8. Epub 2002 Dec 7.PMID: 12473658