Goat anti-PRKACA Antibody

Pep ELISA, WB

Goat anti-PRKACA Antibody
Principle Name: PRKACA
Official Symbol: PRKACA
All Name and Symbols: PRKACA; protein kinase, cAMP-dependent, catalytic, alpha; PKACA; PPNAD4; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha; protein kinase A catalytic subunit
Accession Number(s): NP_002721.1; NP_997401.1; NP_001291278.1
Peptide Sequence: ESPAQNTAHLDQ, is from internal region (near N Terminus)
Human Gene ID(s): 5566
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_002721.1; NP_997401.1; NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 48kDa band observed in lysates of cell line HeLa and MCF7 (calculated MW of 49.1kDa according to NP_002721.1). Recommended concentration: 1-3ug/ml.