Goat anti-HSPA5 / GRP78 Antibody

Goat anti-HSPA5 / GRP78 Antibody
Principle Name: HSPA5 / GRP78
Official Symbol: HSPA5
All Name and Symbols: HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); BIP; GRP78; HEL-S-89n; MIF2; 78 kDa glucose-regulated protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; immunoglobu
Accession Number(s): NP_005338.1
Peptide Sequence: ESHQDADIEDFK, is from internal region (near C Terminus)
Human Gene ID(s): 3309
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IF, WBPeptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 75kDa band observed in lysates of cell lines HepG2 and MCF7 and in Mouse Liver lysates, and approx. 80kDa band in Mouse Testis and Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1, Mouse NP_071705.3 and Rat
Reference(s): Reid SP, Shurtleff AC, Costantino JA, Tritsch SR, Retterer C, Spurgers KB, Bavari S. HSPA5 is an essential host factor for Ebola virus infection. Antiviral research 2014 Sep 109 : 171-4.PMID: 25017472