Goat anti-Caveolin 1, Biotinylated Antibody

Goat anti-Caveolin 1, Biotinylated Antibody
Principle Name: Caveolin 1, Biotinylated
Official Symbol: CAV1
All Name and Symbols: CAV1; caveolin 1; BSCL3; CGL3; LCCNS; MSTP085; PPH3; VIP21; caveolin 1, caveolae protein, 22kDa; cell growth-inhibiting protein 32
Accession Number(s): NP_001744.2; NP_001166366.1
Peptide Sequence: DELSEKQVYDAH., is from internal region
Human Gene ID(s): 857
Non-Human GeneID(s): 12389 (mouse); 25404 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001744.2; NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, ICC
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 22+24kDa band observed in Human spleen lysates (calculated MW of 20.5kDa according to NP_001744.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Reference(s): Cavallo-Medved D, Mai J, Dosescu J, Sameni M, Sloane BF. Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells. Journal of cell science 2005 Apr 118 (Pt 7): 1493-503.PMID: 15769846