Goat anti-TCF3 / ITF1, Biotinylated Antibody

Pep ELISA, WB, IHC

Goat anti-TCF3 / ITF1, Biotinylated Antibody
Principle Name: TCF3 / ITF1, Biotinylated
Official Symbol: TCF3
All Name and Symbols: TCF3; transcription factor 3; E2A; E47; ITF1; TCF-3; VDIR; bHLHb21; NOL1-TCF3 fusion; VDR interacting repressor; class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; immunoglobulin transcription factor 1; kappa-E2-binding factor; nega
Accession Number(s): NP_003191.1
Peptide Sequence: KAPRARTSPDEDED., is from internal region
Human Gene ID(s): 6929
Important Comments: This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml.
Reference(s): Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. The Journal of biological chemistry 2004 Oct 279 (43): 45028-35.PMID: 15310760