Goat anti-Aspartyl-tRNA Synthetase (N terminus) Antibody

Pep ELISA, WB

Goat anti-Aspartyl-tRNA Synthetase (N terminus) Antibody
Principle Name: Aspartyl-tRNA Synthetase (N terminus)
Official Symbol: Dars
All Name and Symbols: Dars; aspartyl-tRNA synthetase; 5730439G15Rik; aspRS; aspartyl-tRNA synthetase, cytoplasmic
Accession Number(s): NP_803228.2; NP_663482.2
Peptide Sequence: SRKGQEKPREIVD, is from N Terminus
Non-Human GeneID(s): 226414 (mouse);
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_803228.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 55kDa band observed in Mouse fetal Brain lysates (calculated MW of 57.1kDa according to NP_803228.2). Recommended concentration: 0.3-1ug/ml.
Reference(s): Taft RJ, Vanderver A, Leventer RJ, Damiani SA, Simons C, Grimmond SM, Miller D, Schmidt J, Lockhart PJ, Pope K, Ru K, Crawford J, Rosser T, de Coo IF, Juneja M, Verma IC, Prabhakar P, Blaser S, Raiman J, Pouwels PJ, Bevova MR, Abbink TE, van der Knaap MS, Mutations in DARS cause hypomyelination with brain stem and spinal cord involvement and leg spasticity. American journal of human genetics 2013 May 92 (5): 774-80.PMID: 23643384