Description |
Goat anti-Aconitase 2 (aa541-555), Biotinylated Antibody Principle Name: Aconitase 2 (aa541-555), Biotinylated Official Symbol: ACO2 All Name and Symbols: ACO2; aconitase 2; ACONM; HEL-S-284; ICRD; OCA8; OPA9; aconitase 2, mitochondrial; citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; mitochondrial aconitase Accession Number(s): NP_001089.1 Peptide Sequence: CQDTYQHPPKDSSGQH., is from internal region Human Gene ID(s): 50 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, Tested Species: Human, Mouse, Rat, Pig Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml. See Reference(s): Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nature cell biology 2002 Sep 4 (9): 674-80..PMID: 12198491 |