Sheep Polyclonal anti-mouse Sonic Hedgehog (Shh C-terminus)

Sheep Polyclonal anti-mouse Sonic Hedgehog (Shh C-terminus)
Immumogen: E. coli-derived recombinant mouse Sonic Hedgehog/Shh C Terminus
Preparation: This antibody was produced from a sheep immunized with purified mouse Sonic Hedgehog/Shh CTerminus
protein.
Formulation and Storage: The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity: Detects mouse Shh C-terminal peptide in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse (rm) Dhh C-terminal peptide is observed and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198) is observed. Detects human and mouse Sonic Hedgehog protein in Western blots. Shh C-terminal peptide specific IgG was purified by first passing the goat sera over a mouse Shh N-terminal peptide affinity column. The unbound fraction from the mouse Shh N-terminal peptide affinity column was subsequently purified using a mouse Shh C-terminal peptide affinity column.
Reconstitution: Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications:
A.:WB-(1 to 1000-2000)
B.: IHC(Paraffin/Frozen)-(1 to 50-100)
C.:
D.: