Mouse Monoclonal Antibody to AURKB

Mouse IgG1

Enterez GeneID: 9212
General Information: AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39kDa), localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases
Immunogen: Purified recombinant fragment of AURKB expressed in E. Coli.
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Ascitic fluid containing 0.03% sodium azide.
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000
References: 1. Azoitei N, et al. Biochemistry. 2005.14; 44(23): 8326-36. ; 2. Kumar KA, et al. Neurosci Lett. 2003.10; 340(2): 139-42. ; 3. Peet GW, et al. J Biol Chem. 1999 Nov 12; 274(46): 32655-61. ;