Mouse Monoclonal Antibody to Cytokeratin 5

Mouse IgG1

Enterez GeneID: 3852
General Information: CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
Immunogen: Purified recombinant fragment of CK5 expressed in E. Coli.
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Ascitic fluid containing 0.03% sodium azide.
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: 1 to 200 - 1 to 1000
References: 1. Mizuguchi, G. et al. Science 2004 303, 343-348. ; 2. Jin, J. et al. Trends Biochem. Sci. 2005 30, 680-687. ; 3. Raisner, R.M. and Madhani, H.D. Curr. Opin. Genet. 2006 Dev. 16, 119-124. ;