Mouse Monoclonal Antibody to AKT1

Mouse IgG1

MW: 55.7kDa
Enterez GeneID: 207
General Information: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. ;
Immunogen: Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Purified antibody in PBS with 0.05% sodium azide
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: ; ICC: 1 to 200 - 1 to 1000; FCM: 1 to 200 - 1 to 400
References: 1.Placenta. 2015 Apr; 36 Suppl 1:S60-5. ; 2.Int J Gynecol Cancer. 2014 Nov; 24(9):1709-14.;