CD14 Mouse Recombinant

Lyophilized powder at room temperature.

The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.
Physical Appearance and Stability: Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Mouse CD14 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Application: ELISA. Inhibition Assays. Western Blotting.
Solubility: It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.