Globular Adiponectin Human Recombinant

Lyophilized powder at room temperature

gAcrp30 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 145 amino acids and having a molecular mass of 16.7kDa. The gAcrp30 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.
Physical Appearance and Stability: For long term, store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time two weeks.
Formulation and Purity: Sterile filtered and lyophilized from 1mg/ml in 10mM Tris pH-8 & 0.75mM DTT. Greater than 95% as determined by SDS-PAGE.
Solubility: We recommended reconstituting gAcrp30 at a concentration of 0.1-1mg per ml with 5mM Tris pH-8 and 0.75mM DTT which can then be further diluted to other aqueous solutions.
Biological Activity: gAcrp30 activity is determined by its ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 0.36-0.54 ug/ml.