Interleukin-11 Human Recombinant, Pichia

Lyophilized powder at room temperature.

IL11 Human Recombinant produced in Pichia Pastoris is a single, non-glycosylated, Polypeptide chain containing 177 amino acids (it differs from the 178 amino acid length of the native IL11 only in lack of the N-terminal praline residue) and having a molecular mass of 19kDa.The IL11 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly.
Source: Pichia Pastoris.
Physical Appearance and Stability: Lyophilized IL11 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL11 should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: IL11 was lyophilized after extensive dialysis against 20mM PB, pH7.0 and 2% Glycine buffer. Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Interleukin -11 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine 7TD1 was found to be less then 0.2ng/ml, corresponding to a Specific Activity of 8, 000, 000 IU/ mg.