Interleukin-1 alpha Rat Recombinant

Lyophilized powder at room temperature.

Interleukin-1A Rat Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 155 amino acids and having a molecular mass of 17703 Dalton. The IL-1A is purified by proprietary chromatographic techniques.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-His-Ser-Phe.
Physical Appearance and Stability: Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL1A should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50mM Tris-HCl, pH=8. Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a Specific Activity of 200, 000, 000IU/mg.