Interleukin-1 alpha Human Recombinant, HEK

Lyophilized powder at room temperature.

Interleukin-1 alpha Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation.The IL1A is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized IL-1A although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL1A should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The IL-1A protein was lyophilized from a 0.2um filtered
solution of PBS pH 7.4 with 10% trehalose as protectant. Greater than 97.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized IL1A in
deionized water to a stock solution of 0.5mg/ml.
Biological Activity: Assay # 1: The biological activity was measured by its
binding ability in a functional ELISA, the ED50 is typically 0.5-5 ug/ml. Assay # 2: The biological activity was determined by the
dose-dependent stimulation of the proliferation of mouse D10S cells, the ED50
is typically less than 1pg/ml, corresponding to a specific activity of > 1x109
unit/mg.