Leukemia Inhibitory Factor Human Recombinant, HEK

Lyophilized powder at room temperature.

Leukemia Inhibitory Factor Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 19.9kDa due to glycosylation.The LIF is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution LIF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The LIF protein was lyophilized from 1mg/ml in PBS pH 7.4. Greater than 95.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized LIF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Biological Activity: The biological activity was determined by its ability to stimulate the proliferation of Human TF-1 cells, the ED50 is typically less than 0.1ng/ml.