Tumor Necrosis Factor-Alpha Human Recombinant, His Tag

Lyophilized powder at room temperature.

Tumor Necrosis Factor-a Human Recombinant His produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 18.3kDa with an amino-terminal hexahistidine tag. The TNF-alpha His is purified by standard chromatographic techniques.
Physical Appearance and Stability: Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNF-a should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: TNF-a His is supplied in 1xPBS. Greater than 97.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TNF-a in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).