Tumor Necrosis Factor Receptor Type 2 Mouse Recombinant

Lyophilized powder at room temperature.

TNFR2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20kDa.The TNFR2 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.
Physical Appearance and Stability: Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: TNFR2 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4. Greater than 97.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by its ability to inhibit the TNF-? mediated cytotoxicity in the L-929 cells is less than 0.2ug/ml, corresponding to a specific activity of > 5000IU/mg in the presence of 0.25ng/mL of rHuTNF-a.