TEK Tyrosine Kinase Endothelial Fc Chimera Human Recombinant

Lyophilized powder at room temperature.

Soluble TEK Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 730 amino acids and having a total molecular mass of 250 kDa. Human TIE-2/Fc monomer has a calculated molecular mass of approximately 125 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions.The TEK Fc Chimera is purified by proprietary chromatographic techniques.
Source: Insect Cells.
Physical Appearance and Stability: Lyophilized sTIE-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TEK should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: TEK Fc Chimera was lyophilized from a concentrated (1mg/ml) sterile solution containing 1xPBS. Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TIE-2 Fc Chimera in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.