Glutathione S-Transferase Recombinant, His Tag

Lyophilized powder at room temperature.

Recombinant Schistosoma japonicum GST full length protein contains 244 amino acids(1-224 a.a.) expressed in E.coli, having a molecular mass of 28.3kDa. The GST protein is fused to a 20 amino acids His-Tag at N-terminus. The GST protein is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: GST is supplied in PBS pH 7.4 & 10% glycerol. Greater than 95% as determined by SDS-PAGE.
Biological Activity: 2.8-3.3 units/mg, & is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH-6.5 at 25C.