Vascular Endothelial Growth Factor Receptor-3 Human Recombinant

Lyophilized powder at room temperature.

Soluble FLT4 Human Recombinant fused with a carboxy-terminal 6X histidine-tag produced in baculovirus is a monomeric, glycosylated, polypeptide containing the extracellular part, 25-774 amino acids and having a total molecular mass of 120 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for ligand binding.
The FLT4 is purified by proprietary chromatographic techniques.
Source: Insect Cells.
Physical Appearance and Stability: Lyophilized FLT4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FLT4 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: FLT4 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1xPBS. Greater than 90.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized FLT4 in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human VEGFR-3/FLT-4 at 5 ug/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml.