Granulocyte-Colony Stimulating Factor Human Recombinant

Lyophilized powder at room temperature.

Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic techniques.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly.
Physical Appearance and Stability: Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GCSF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4. Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized GCSF in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100, 000, 000 IU/mg.