Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant

Lyophilized powder at room temperature.

GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.
Amino Acid Sequence:APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK.
Physical Appearance and Stability: Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4. Greater than 96.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized GMCSF in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.