Erythropoietin-alpha Human Recombinant, Sf9

Lyophilized powder at room temperature.

Erythropoietin-alpha Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 174 amino acids (28-193a.a.) and having a molecular mass of 19.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).EPO-a is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Amino Acid Sequence:APPRLICDSR
VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL
LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR
GKLKLYTGEA CRTGDRLEHH HHHH.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks.Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: EPO a protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. Greater than 95.0% as determined by SDS-PAGE.
Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is = 0.5 ng/ml.