Fibroblast Growth Factor Acidic Bovine

Product Name: Fibroblast Growth Factor Acidic Bovine
Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Description: Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al²). The FGF acidic is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P03968
Amino Acid Sequence:
Source: Bovine Brain.
Physical Appearance and Stability: Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18C. Upon reconstitution aFGF should be stored at 4C between 2-3 weeks and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: Each 5ug aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane. Greater than 90%.
Application:
Solubility: It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Biological Activity: Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.