Description |
Product Name: Fibroblast Growth Factor-8 Human Recombinant Synonyms: FGF8B, FGF-8B, FGF8-B, KAL6, HBGF-8, HBGF8, AIGF, HBGF-8, MGC149376, fibroblast growth factor 8. Description: FGF 8 Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 194 amino acids and having a total molecular mass of 22.5kDa. Uniprot Accesion Number: P55075 Amino Acid Sequence:MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGDPFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized FGF 8 although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution FGF 8 should be stored at 4?C between 2-7 days and for future use below -18?C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: Lyophilized from a concentrated (1mg/ml) solution containing 5mM Na3PO4 and 50mM NaCl, pH 7.5. Greater than 97.0% as determined by analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized FGF 8 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, as determined by its ability to induce proliferation of mouse 3T3 fibroblasts, is 5-7ng/ml, corresponding to a specific activity of 2x105units/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |