Growth Hormone Human Recombinant, Plant

Lyophilized powder at room temperature.

GH human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 205 amino acids (molecular formula C1025H1570N280O306S7) and 6-His-tag at the N-terminal having the total molecular mass of 22.9kDa.
Amino Acid Sequence:HHHHHHFPTI PLSRPFDNAM LRAHRLHQLA FDTYQEFEEA YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGFAG
Source: Nicotiana benthamiana plant
Physical Appearance and Stability: Lyophilized GH although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GH should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: Lyophilized from 1mg/ml solution in PBS 0.05M buffer at pH 7.5. Greater than 97.0% as determined by Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized GH in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 < 0.04-0.1 ng/mL.