Growth Hormone Placental 20kDa Human Recombinant

Lyophilized powder at room temperature.

Growth Hormone Placental 20kDa Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. Growth Hormone 20K placental is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs. GH 20K placental is purified by proprietary chromatographic techniques.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile.
Physical Appearance and Stability: Lyophilized Growth Hormone 20K Placental although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization GH 20K pl can be stored at 4C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: pGH 20kDa was lyophilized from a concentrated (1mg/ml) solution with0.0045mM NaHCO3 previously adjusted pH 11. Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Placental Growth Hormone in 0.4% NaHCO3or water adjusted to pH 11, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.