Placental Ovine Growth Hormone Recombinant

Lyophilized powder at room temperature.

Placental Growth Hormone Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21918 Dalton. The GH Ovine Recombinant is purified by proprietary chromatographic techniques.Placental Growth Hormone differs from pituitary Ovine Growth Hormone by two amino acids G9R/G63S. Placental Ovine Growth Hormone possesses higher biological activity as compared to pituitary Ovine GH.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.
Physical Appearance and Stability: Lyophilized Ovine Placental Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GH Ovine Placental should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH-9. Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Growth Hormone in sterile 0.4% NaHCO3 pH-9 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: Ovine placental Grwoth Hormone is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).