Growth Hormone Mutant Zebrafish Recombinant

Lyophilized powder at room temperature.

Somatotropin Zebrafish Mutant G113R Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 185 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21.18 kDa.
The Zebrafish Mutant G113R Recombinant is purified by proprietary chromatographic techniques.
Amino Acid Sequence:The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Gln-Arg-Leu-Phe-Asn.
Physical Appearance and Stability: Lyophilized Growth-Hormone although stable at room temperature for at least two weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization GH can be stored at 4C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.5% NaHCO3 pH-8. Greater than 99.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Zebrafish Mutant G113R in 0.4% NaHCO3 or water adjusted to pH-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.