Insulin-Like Growth Factor 1 Human Recombinant

Lyophilized powder at room temperature.

Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa.IGF-I is purified by proprietary chromatographic techniques.
Amino Acid Sequence:GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.
Physical Appearance and Stability: Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGF1 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a 0.2um filtered concentrated solution in 20mM PB, pH 7.0, 130mM NaCl. Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1x106 units/mg.