Keratinocyte Growth Factor-2 Human Recombinant, His Tag

Lyophilized powder at room temperature.

KGF 2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (38-208) and having a molecular mass of 22.0kDa.KGF 2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMQALGQ DMVSPEATNS SSSSFSSPSS AGRHVRSYNH LQGDVRWRKL FSFTKYFLKI EKNGKVSGTK KENCPYSILE ITSVEIGVVA VKAINSNYYL AMNKKGKLYG SKEFNNDCKL KERIEENGYN TYASFNWQHN GRQMYVALNG KGAPRRGQKT RRKNTSAHFL PMVVHS
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The KGF 2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl 2mM DTT, 2mM EDTA and 50% glycerol. Greater than 95% as determined by SDS-PAGE.