Osteoprotegerin Human Recombinant, His Tag

Lyophilized powder at room temperature

Recombinant Human OCIF produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing amino acids 201-401 and having a molecular mass of 31 kDa which includes a 4 kDa His tag.The OPG is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution OCIF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT. Greater than 80.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application: 1. Positive control for Western blot.2. Antibody production. 3. Protein assay.
Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.