Vascular Endothelial Growth Factor Human Recombinant, CHO

Lyophilized powder at room temperature.

Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions. The VEGF is purified by proprietary chromatographic techniques.
Source: Chinese Hamster Ovarian Cell.
Physical Appearance and Stability: Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution VEGF Human Recombinant should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4. Greater than 97.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The protein was tested in HUVEC cells, the ED50 for this effect was found to be 2-6ng/ml.