Beta Nerve Growth Factor Mouse Recombinant

Lyophilized powder at room temperature.

Recombinant Mouse b-NGF produced in E.Coli is a noncovalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical chains of 120 amino acids each and having a molecular mass of 13, 471 Dalton each.The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology.
Amino Acid Sequence:MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G.
Physical Appearance and Stability: Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Murine NGF-Beta should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: The Recombinant Mouse b-NGF was lyophilized without additives. Greater than 98% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE .
Solubility: It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The Mouse b-NGF activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is 0.2 ng/ml, corresponding to a Specific Activity of 5, 000, 000 units/mg.