Complement C1q Tumor Necrosis Factor-Related Protein 3 Human Recombinant

Lyophilized powder at room temperature.

C1QTNF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 234 amino acids and having a molecular mass of 25.4 kDa. The protein contains an extra 10 aa His tag at N-terminus. The C1QTNF3 amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q9BXJ4 amino acids 23¿246. The C1QTNF3 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MKHHHHHHAS QDEYMESPQT GGLPPDCSKC CHGDYSFRGY QGPPGPPGPP GIPGNHGNNG NNGATGHEGA KGEKGDKGDL GPRGERGQHG PKGEKGYPGI PPELQIAFMA SLATHFSNQN SGIIFSSVET NIGNFFDVMT GRFGAPVSGV YFFTFSMMKH EDVEEVYVYL MHNGNTVFSM YSYEMKGKSD TSSNHAVLKL AKGDEVWLRM GNGALHGDHQ RFSTFAGFLLFETK.
Physical Appearance and Stability: Store lyophilized C1QTNF3 at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted C1QTNF3 can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C.
Formulation and Purity: Human C1QTNF3 was filtered (0.4um) and lyophilized in 0.5 mg/ml in 0.05M Acetate buffer pH4. The purity of C1QTNF3 is greater than 95% as determined by SDS PAGE.
Solubility: It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of the protein is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.