Alpha Fetoprotein Human Recombinant

Lyophilized powder at room temperature.

AFP produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 597 amino acids (19-609a.a.) and having a molecular mass of 67.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
AFP is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Amino Acid Sequence:RTLHRNEYGI ASILDSYQCT AEISLADLAT IFFAQFVQEA TYKEVSKMVK
DALTAIEKPT GDEQSSGCLE NQLPAFLEEL CHEKEILEKY GHSDCCSQSE EGRHNCFLAH KKPTPASIPL
FQVPEPVTSC EAYEEDRETF MNKFIYEIAR RHPFLYAPTI LLWAARYDKI IPSCCKAENA
VECFQTKAAT VTKELRESSL LNQHACAVMK NFGTRTFQAI TVTKLSQKFT
KVNFTEIQKL VLDVAHVHEH CCRGDVLDCL QDGEKIMSYI CSQQDTLSNK ITECCKLTTL ERGQCIIHAE
NDEKPEGLSP NLNRFLGDRD FNQFSSGEKN IFLASFVHEY SRRHPQLAVS VILRVAKGYQ
ELLEKCFQTE NPLECQDKGE EELQKYIQES QALAKRSCGL FQKLGEYYLQ
NAFLVAYTKK APQLTSSELM AITRKMAATA ATCCQLSEDK LLACGEGAAD IIIGHLCIRH EMTPVNPGVG
QCCTSSYANR RPCFSSLVVD ETYVPPAFSD DKFIFHKDLC QAQGVALQTM KQEFLINLVK
QKPQITEEQL EAVIADFSGL
LEKCCQGQEQ EVCFAEEGQK LISKTRAALG VHHHHHH.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: AFP protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol. Greater than 95.0% as determined by SDS-PAGE.