Staphylococcal Protein-A Recombinant, His Tag

Lyophilized powder at room temperature.

SPA Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain fused with 6xHis tag at C-terminus. SPA is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 306 amino acids and having a molecular mass of 34.7kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
Amino Acid Sequence:MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH.
Physical Appearance and Stability: Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution SPA should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: SPA protein was lyophilized with no additives. Greater than 97.0% as determined by: (a) Analysis by HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized SPA in sterile 18MO-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.