Small Nuclear Ribonucleoprotein 70kDa Human Recombinant

Lyophilized powder at room temperature.

Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).
Physical Appearance and Stability: Store at 4° C if entire vial will be used within 2-4 weeks. Store, frozen at -20° C for longer periods of time. Avoid multiple freeze-thaw cycles.
Formulation and Purity: U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS. Greater than 90% as determined by SDS-PAGE.
Application: Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).