Comparison

Anti-GFP Antibody

Item no. 75-131-020
Manufacturer Antibodies Incorporated
Amount 20 uL
Quantity options 100 uL 20 uL
Category
Format Liquid
Applications WB, IP, IHC, ICC, ELISA, CHIP
Clone N86/8
Specific against other
Host Mouse
Isotype IgG2a
Conjugate/Tag Unconjugated
Alias Green fluorescent protein
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibody
Manufacturer - Targets
GFP
Country of Origin
United States
Shipping Temperature
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Molecular Weight
27 kDa
Manufacturer - Research Area
Anti-Tag Antibodies
Expiration
24 months from date of receipt
Immunogen
Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli
Immunogen Species
Jellyfish
Description
Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/8. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB, ELISA, CHiP.
Target Description
Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.
Clonality
Monoclonal
Manufacturer - Specificity
Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)
Concentration
1 mg/mL
Form
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.57
Production Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Quality Control
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
Quality Control: Application
ICC
Dilution Range: WB
1:1000
Dilution Range: IHC
1:1000
Dilution Range: ICC
1:500
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Manufacturer - Statement
We produce our GFP mouse monoclonal primary antibody from hybridoma clone N86/8. It is great in IHC, ICC, IP, WB, ELISA, CHiP and is purified by Protein A chromatography.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 uL
Available: In stock
available

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