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Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®

Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®

Item no.	BOS-A01413-1-Biotin
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Antibodies Western Blot Immunofluorescence Immunocytochemistry Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies By Organ Colorectal Cancer Immunochemistry
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, IF, ICC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Biotin
Citations	1. Hanukoglu, I., Hanukoglu, A. Epithelial sodium channel (ENaC) family: phylogeny, structure-function, tissue distribution, and associated inherited diseases. Gene 579: 95-132, 2016. 2. Iwai, N., Baba, S., Mannami, T., Ogihara, T., Ogata, J. Association of a sodium channel alpha subunit promoter variant with blood pressure. J. Am. Soc. Nephrol. 13: 80-85, 2002.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Amiloride-sensitive sodium channel subunit alpha,Alpha-NaCH,Epithelial Na (+) channel subunit alpha,Alpha-ENaC,ENaCA,Nonvoltage-gated sodium channel 1 subunit alpha,SCNEA,SCNN1A,SCNN1
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	76 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml Immunocytochemistry/ Immunofluorescence, 2μg/ml
Gene Name	SCNN1A
Gene Full Name	sodium channel epithelial 1 alpha subunit
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	The SCNN1A gene encodes the alpha subunit of the epithelial sodium channel (ENaC), a constitutively active channel that allows the flow of sodium ions from the lumen into epithelial cells across the apical cell membrane. The ENaC channel, which is regulated by the renin-angiotensin-aldosterone system, has a central role in the regulation of extracellular fluid volume and blood pressure. The other subunits are encoded by the beta (SCNN1B), gamma (SCNN1G), and delta (SCNN1D) genes. This SCNN1A gene is mapped to 12p13.31. Mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human SCNN1A, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat SCNN1A.
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, NK Cells, Stem Cells
Protein Function	Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
Subcellular Localization	Apical cell membrane.
Description	Boster Bio Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® catalog # A01413-1. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244). Expressed in kidney (at protein level). In the respiratory tract, expressed in the bronchial epithelium (at protein level). Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244). in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub- layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590). Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590). Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590). Expressed by eccrine sweat glands (at protein level) (PubMed:28130590). In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590). Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart (PubMed:9575806).
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A01413-1-Biotin-datasheet.pdf

BOS-A01413-1-Biotin-safety-datasheet.pdf