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Anti-GPCR TGR5/GPBAR1 Antibody Picoband®

Anti-GPCR TGR5/GPBAR1 Antibody Picoband®

Item no.	BOS-A01958-2-Dylight488
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Metabolism Cholesterol & Lipid Metabolism Antibodies By Cell Type Flow Cytometry Western Blot ELISA Primary Antibodies Immunostaining Other Lipid\Cholesterol Metabolism Modulators
Type	Antibody
Format	Lyophilized
Applications	WB, FC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	DyLight 488
Citations	1. Maruyama, T., Miyamoto, Y., Nakamura, T., Tamai, Y., Okada, H., Sugiyama, E., Nakamura, T., Itadani, H., Tanaka, K. Identification of membrane-type receptor for bile acids (M-BAR). Biochem. Biophys. Res. Commun. 298: 714-719, 2002. 2. Watanabe, M., Houten, S. M., Mataki, C., Christoffolete, M. A., Kim, B. W., Sato, H., Messaddeq, N., Harney, J. W., Ezaki, O., Kodama, T., Schoonjans, K., Bianco, A. C., Auwerx, J. Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation. Nature 439: 484-489, 2006. 3. Yoneno, K., Hisamatsu, T., Shimamura, K., Kamada, N., Ichikawa, R., Kitazume, M. T., Mori, M., Uo, M., Namikawa, Y., Matsuoka, K., Sato, T., Koganei, K., Sugita, A., Kanai, T., Hibi, T. TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease. Immunology 139: 19-29, 2013.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,IGFBP5,IBP5
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Observed Molecular weight	45 kDa
Calculated Molecular weight	30570 MW
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5 μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/ml
Gene Name	GPBAR1
Gene Full Name	Insulin-like growth factor-binding protein 5
Background	The G protein-coupled bile acid receptor 1 (GPBAR1) also known G-protein coupled receptor 19 (GPCR19), membrane-type receptor for bile acids (M-BAR) or TGR5 as is a protein that in humans is encoded by the GPBAR1 gene. This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Immunogen	E.coli-derived human GPCR TGR5/GPBAR1 recombinant protein (Position: N76-S310).
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway
Protein Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subcellular Localization	Secreted.
Description	Boster Bio Anti-GPCR TGR5/GPBAR1 Antibody Picoband® catalog # A01958-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Osteosarcoma, and at lower levels in liver, kidney and brain.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A01958-2-Dylight488-datasheet.pdf

BOS-A01958-2-Dylight488-safety-datasheet.pdf