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Anti-ALKBH5 Antibody Picoband®

Anti-ALKBH5 Antibody Picoband®

Item no.	BOS-A03360-HRP
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Neuroscience Targets for Rodent Neurosciences Antibodies Flow Cytometry Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Primary Antibody
Format	Lyophilized
Applications	WB, IF, ICC, ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	HRP
Citations	1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/12/2010. 2. Tsujikawa, K., Koike, K., Kitae, K., Shinkawa, A., Arima, H., Suzuki, T., Tsuchiya, M., Makino, Y., Furukawa, T., Konishi, N., Yamamoto, H. Expression and sub-cellular localization of human ABH family molecules. J. Cell. Molec. Med. 11: 1105-1116, 2007. 3. Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vagbo, C. B., Shi, Y., Wang, W.-L., Song, S.-H., Lu, Z., Bosmans, R. P. G., and 16 others. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Molec. Cell 49: 18-29, 2013.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RNA demethylase ALKBH5,Alkylated DNA repair protein alkB homolog 5,Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5,ALKBH5,ABH5,OFOXD1
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	52 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.25μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human ELISA, 0.1-0.5μg/ml
Gene Name	ALKBH5
Gene Full Name	alkB homolog 5, RNA demethylase
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N (6)-methyladenosine (m (6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m (6)A from mRNAs.
Immunogen	E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180).
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Adaptive Immunity, Immunology, Lipid Signaling, Signal Transduction, Signaling Pathway, T Cells
Protein Function	Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N6-methyladenosine in single-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
Subcellular Localization	Nucleus speckle.
Description	Boster Bio Anti-ALKBH5 Antibody Picoband® catalog # A03360. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A03360-HRP-datasheet.pdf

BOS-A03360-HRP-safety-datasheet.pdf