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Anti-MED14 Antibody Picoband®

Anti-MED14 Antibody Picoband®

Item no.	BOS-A04799-2-APC
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Antibodies Western Blot ELISA Primary Antibodies By Organ Thyroid Cancer Immunostaining
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	APC
Citations	1. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. Nature. 397 (6718): 446–50. 2. Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K (Aug 1998). Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method. Genomics. 49 (2): 237–46.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	160 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml
Gene Name	MED14
Gene Full Name	mediator complex subunit 14
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Mediator of RNA polymerase II transcription subunit 14 is an enzyme that in humans is encoded by the MED14 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
Immunogen	E. coli-derived human MED14 recombinant protein (Position: D181-D375).
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Epigenetics and Nuclear Signaling, Mediator Complex, Transcription
Protein Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subcellular Localization	Nucleus.
Description	Boster Bio Anti-MED14 Antibody Picoband® catalog # A04799-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Ubiquitous.
Manufacturer - Alias	Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A04799-2-APC-datasheet.pdf

BOS-A04799-2-APC-safety-datasheet.pdf