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Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3)

Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3)

Item no.	BOS-M00229-1-Dylight550
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Antibodies By Cell Type Carcinoma Hematology Lymphoma Myeloma Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Astrocytoma
Type	Antibody Monoclonal
Format	Lyophilized
Applications	WB, FC, IHC
Clone	6I3
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b
Conjugate/Tag	Dylight 550
Sensitivity	>5000 cells
Citations	1. Chu-Shore, C. J., Major, P., Montenegro, M., Thiele, E. Cyst-like tubers are associated with TSC2 and epilepsy in tuberous sclerosis complex. Neurology 72: 1165-1169, 2009. 2. Jansen, F. E., Braams, O., Vincken, K. L., Algra, A., Anbeek, P., Jennekens-Schinkel, A., Halley, D., Zonnenberg, B. A., van den Ouweland, A., van Huffelen, A. C., van Nieuwenhuizen, O., Nellist, M. Overlapping neurologic and cognitive phenotypes in patients with TSC1 or TSC2 mutations. Neurology 70: 908-915, 2008.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Tuberin,Tuberous sclerosis 2 protein,TSC2,TSC4
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	280 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Gene Name	TSC2
Gene Full Name	tuberous sclerosis 2
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Tuberous sclerosis 2 protein, also known as TSC2 or Tuberin is a protein that is in humans. The chromosomal location of this gene is 16p13.3. Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. This gene involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. It stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro.
Immunogen	E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807). Human Tuberin shares 94% and 90% amino acid (aa) sequence identity with mouse and rat Tuberin, respectively.
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Manufacturer - Research Category	Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways and Processes, Signal Transduction, Signaling Pathway
Protein Name	Glial fibrillary acidic protein
Protein Function	In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a activator of the protein kinase activity of mTORC1 (PubMed:15340059). May also play a role in microtubule-mediated protein transport (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).
Subcellular Localization	Cytoplasm; Membrane; Peripheral membrane protein
Description	Boster Bio Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) catalog # M00229-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-M00229-1-Dylight550-datasheet.pdf

BOS-M00229-1-Dylight550-safety-datasheet.pdf