Anti-5 Lipoxygenase/ALOX5 Antibody Picoband®

Item no.	BOS-PA1644-Dylight550
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug 100 ug 100 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug/vial 100 ug
Category	Oncology Neuroscience Metabolism Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Adenocarcinoma Immunohistochemistry Western Blot Enzymes Primary Antibodies Leukotriene Metabolism
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Dylight 550
Sensitivity	>5000 cells
Sequence	Belongs to the lipoxygenase family.
Citations	1. Aliberti, J., Serhan, C., Sher, A. Parasite-induced lipoxin A4 is an endogenous regulator of IL-12 production and immunopathology in Toxoplasma gondii infection. J. Exp. Med. 196: 1253-1262, 2002. 2. Bafica, A., Scanga, C. A., Serhan, C., Machado, F., White, S., Sher, A., Aliberti, J. Host control of Mycobacterium tuberculosis is regulated by 5-lipoxygenase-dependent lipoxin production. J. Clin. Invest. 115: 1601-1606, 2005. 3. Funk CD, Hoshiko S, Matsumoto T, Rdmark O, Samuelsson B (April 1989). Characterization of the human 5-lipoxygenase gene. Proc. Natl. Acad. Sci. U.S.A. 86 (8): 2587–91.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Arachidonate 5-lipoxygenase,5-LO,5-lipoxygenase,1.13.11.34,ALOX5,LOG5
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	78 kDa
Calculated Molecular weight	77983 MW
Clonality	Polyclonal
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Western blot, 0.1-0.5μg/ml, Human
Gene Name	ALOX5
Gene Full Name	Arachidonate 5-lipoxygenase
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	ALOX5 (ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO (5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5, different from the related rat and mouse sequences by two amino acids.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Energy Metabolism, Energy Transfer Pathways, Heart Disease, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction
Protein Name	Arachidonate 5-lipoxygenase(5-LO/5-lipoxygenase)
Protein Function	Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Subcellular Localization	Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Description	Boster Bio Anti-5 Lipoxygenase/ALOX5 Antibody catalog # PA1644. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Sequence Similarities	Belongs to the lipoxygenase family.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PA1644-Dylight550-datasheet.pdf

BOS-PA1644-Dylight550-safety-datasheet.pdf